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ECL DIRECT NUCLEIC ACID

ECL DIRECT NUCLEIC ACID
ECL DIRECT NUCLEIC ACID, 1 EA, For fast and easy detection of medium- to high-target amounts in applications, Category: Amersham Blotting & Labeling & Detection, Nucleic Acid Labeling and Detection, Nonradioactive Labeling and Detection, ECL Direct Labeling and Detection System ECL Direct Labeling and Detection System Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL Direct, Hybond N+, and Hyperfilm ECL. Eliminates handling, waste, and regulatory issues associated with the use of radioactivity. No need to strip blots before reprobing. For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses. Consistent results combining strong signals with very low backgrounds. ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol. Each system includes the following reagents, sufficient for labeling 5 to 10 µg nucleic acid and detecting 2000 to 4000 cm² of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL Detection Reagents, and ECL Gold Hybridization Buffer.

Package Content: 1 PCE

Reference Num.: GERPN3001