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Sera-Mag Magnetic Olido(dt) particles, 1 um, 1% solids, 1 mL, 0.05% Na azide

Sera-Mag Magnetic Olido(dt) particles, 1 um, 1% solids, 1 mL, 0.05% Na azide
Sera-Mag Magnetic Oligo(dt) particles, 1 µm, 1% solids, 1 mL, 0.05% Na azide. Used in molecular biology nucleic acid isolation, research and diagnostic assays. High binding capacity, excellent stability and sensitivity. euro per article Sera-Mag Oligo (dT) Coated Magnetic particles Sera-Mag Oligo(dT)-Coated Magnetic Particles enable convenient mRNA isolation and extraction. Convenient isolation and extraction of mRNA from a variety of sources for downstream applications such as RT-PCR, cDNA library construction, cDNA microarrays, affinity purification, primer extension, and subtractive hybridization. Covalently bound oligo(dT)14 prevents leaching from the particle surface. Very high, specific poly A+ binding capacity ensures maximum extraction of mRNA. Fast reaction kinetics increases throughput and precision, and also enables faster movement through viscous solutions. Encapsulation means no exposed iron and minimized interference with downstream enzymatic applications. Uniform, nominal 1 ?m diameter provides high surface area and excellent lot-to-lot reproducibility. Sera-Mag Oligo(dT) Magnetic Particles bind target mRNA through pairing of the polyadenylated RNA tail found on the 3’ end of mRNA to the covalently bound oligo(dT) groups on the surface of the particles. This binding is easily accomplished using standard hybridization conditions. Convenient and Efficient mRNA Isolation Versatile and efficient methods for mRNA purification are critical, given that eukaryotic mRNA makes up only 1 - 3% of total cellular RNA. Sera-Mag Oligo(dT) Magnetic Particles remove 90% or more of mRNA from total RNA with just one extraction. The core of the particle is made by a free radical emulsion polymerization of styrene and acid monomer. One layer of magnetite is coated onto this core, while the surface is modified to minimize non-specific binding of proteins. Oligo(dT) groups are then covalenty bound to the particle surface. Fast Magnetic Response Times and Stable Physical Integrity

Package Content: 1 PCE

Reference Num.: GE38152103011150