Superose 6 Increase 10/300 GL
Superose 6 Increase 10/300 GL
Pre-packed size exclusion column for small scale (25–500µl) preparative purification and analysis of proteins with molecular weights between 5 000 and 5 000 000. Agarose beads for high pH stability. Superose 6 Increase 10/300 GL Superose 6 Increase 10/300 GL is a versatile, prepacked column for high-resolution size exclusion chromatography in small-scale ( 500 µl sample volume) preparative purification, as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000. Increased resolution compared with Superose 6, for higher purity and improved analysis results. Reduced runtime compared with Superose 6, for rapid results. Versatile use for both preparative and analytical purposes, especially for large proteins and complexes. Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over. Superose 6 Increase is part of the Superdex 200 Increase family, the next generation of size exclusion chromatography (gel filtration) media, offering versatility and higher resolution within a shorter time frame. Superose 6 Increase 10/300 GL column is suitable for small-scale (25 – 500 µl sample volume) preparative purification for obtaining milligram amounts of highly size-homogenous target proteins at the polishing stage of a purification procedure. It is also well-suited for the characterization of large protein complexes and for the study of protein-protein interactions. This size exclusion chromatography (SEC) medium has a very broad fractionation range (Mr 5 000 to 5 000 000) for both proteins and dextrans, making it of special interest to researchers working with protein complexes, membrane proteins, and other macromolecules. It also makes the columns useful as a screening tool to explore the molecular weight distribution of an unknown sample. The increased resolution of Superose 6 Increase compared to Superose 6 is a result of the smaller bead size (mean bead size 8.6 µm) and narrower particle size distribution, giving higher purity and better analysis results. Superose 6 Increase reduces your runtime compared to Superose 6.The high-flow agarose base matrix has excellent pressure/flow properties, which in combination with the smaller bead size, allows for high flow rates and increased resolution. Smaller Bead Size Increases Resolution Superose 6 Increase medium is alkali resistant and supports cleaning-in-place (CIP) procedures, which allows you to use the same column for different proteins with minimal risk for carry-over between samples. Alkali resistance differentiates agarose-based media from silica-based media. In addition, the possibility to clean the medium ensures a long column lifespan. Cleaning-In-Place for Versatility
Package Content: 1 PCE
Reference Num.: GE29091596
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