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HiTrap HIC Selection Kit, 7x1ml

HiTrap HIC Selection Kit, 7x1ml
HITRAP HIC SELECTION KIT, 7X1ML, 7 EA, Select the most appropriate ligand and experimental conditions to take full advantage of hydrophobic inte, Category: Protein Purification, Chromatography Columns and Media, Hydrophobic Interaction, HiTrap HIC Selection Kit HiTrap HIC Selection Kit consists of seven columns prepacked with hydrophobic interaction chromatography media with different characteristics. Screen for the most appropriate HIC media and conditions to use for specific application and development work. Select the most appropriate ligand and experimental conditions to take full advantage of hydrophobic interaction chromatography. Flexible design for use with syringes, single laboratory pump, or chromatography system such as ÄKTA design. High flow rates and high capacities. The kit includes seven HiTrap 1 ml columns containing the following media: Phenyl Sepharose High Performance, Butyl Sepharose High Performance, Phenyl Sepharose 6 Fast Flow (low sub), Phenyl Sepharose 6 Fast Flow (high sub), Butyl-S Sepharose 6 Fast Flow, Butyl Sepharose 4 Fast Flow, and Octyl Sepharose 4 Fast Flow. Phenyl Sepharose High Performance is based on a 34 µm matrix and is an excellent choice for laboratory and intermediate process-scale separations and for final step purifications where high resolution is needed. The degree of substitution gives Phenyl Sepharose High Performance a selectivity similar to that of Phenyl Sepharose 6 Fast Flow (low sub). Butyl Sepharose High Performance is based on a 34 µm matrix. The small beads give high resolution and are an excellent choice for intermediate and final purification steps. The relatively high ligand density enables high dynamic binding capacity for proteins, even at relatively low salt concentration. Phenyl Sepharose 6 Fast Flow (low sub) and Phenyl Sepharose 6 Fast Flow (high sub) are based on a 90 µm matrix. They are an excellent choice for initial and intermediate step purifications requiring a matrix with low to medium hydrophobicity. The availability of two degrees of substitution grades increases the possibility of finding the optimal selectivity and capacity for a given application. Octyl Sepharose 4 Fast Flow, which is also based on a 90 µm matrix, has a different hydrophobic character compared to the phenyl and butyl ligands, and is an important complement to the other hydrophobic matrices.

Package Content: 1 PCE

Reference Num.: GE28411007